Hemin attenuates response of primary rat adipocytes to adrenergic stimulation
نویسندگان
چکیده
Hemin is an activator of heme oxygenase-1 (HO-1), enzyme catalyzing degradation. Up-regulation HO-1 observed in response to various pathological conditions. Moreover, pharmacological activation associated with numerous beneficial effects the organism. was shown exert, among other, anti-diabetic and anti-obesity properties. These are strongly linked adipose tissue. However, direct influence hemin on metabolism fat cells have not been explored. The present study aimed determine short-term primary rat adipocytes. We focused processes directly related lipid accumulation, such as lipogenesis lipolysis. For this purpose, isolated were subjected for 2 h 40 µM hemin, compound insulin-stimulated glucose conversion lipids, lactate release, lipolysis induced by stimuli, also antilipolytic action insulin determined. It that did affect insulin-induced release. significantly decreased stimulated epinephrine. inhibitory effect epinephrine-induced abolished presence SnMP, inhibitor HO-1, which suggests irrespective enzyme. Similar 3 12 mM glucose. reduce when replaced alanine or succinate. Apart from changes epinephrine action, it found lipolytic adipocytes isoproterenol diminished hemin. failed dibutyryl-cAMP (a protein kinase A), forskolin (an adenylate cyclase), DPCPX adenosine A 1 receptor antagonist). Additionally, be insulin, deepened results indicate exposure does metabolism, decrease adrenergic stimulation, reduced release our capable diminishing exaggerated lipolysis, occurs supraphysiological concentrations
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ژورنال
عنوان ژورنال: PeerJ
سال: 2021
ISSN: ['2167-8359']
DOI: https://doi.org/10.7717/peerj.12092